Coding

Part:BBa_K2378001:Design

Designed by: Muhammad Farhan Maulana   Group: iGEM17_ITB_Indonesia   (2017-10-27)


PETase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 639
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We choose synonym mutations for optimized codon preference in E. coli BL21. The original sequence is based on www.uniprot.org/uniprot/A0A0K8P6T7.


Source

Ideonella sakaiensis

References